{"id":7936,"date":"2019-02-25T15:45:16","date_gmt":"2019-02-25T08:45:16","guid":{"rendered":"https:\/\/biotekco.vn\/?p=7936"},"modified":"2019-02-25T15:45:18","modified_gmt":"2019-02-25T08:45:18","slug":"huong-dan-phong-chong-benh-tilapia-lake-virus-tilv-tren-ca-ro-phi","status":"publish","type":"post","link":"https:\/\/hhbio.vn\/?p=7936","title":{"rendered":"H\u01b0\u1edbng d\u1eabn ph\u00f2ng, ch\u1ed1ng b\u1ec7nh Tilapia lake virus (TiLV) tr\u00ean c\u00e1 r\u00f4 phi"},"content":{"rendered":"\n

M\u1ea1ng l\u01b0\u1edbi c\u00e1c Trung t\u00e2m Nu\u00f4i tr\u1ed3ng th\u1ee7y s\u1ea3n khu v\u1ef1c ch\u00e2u \u00c1 – Th\u00e1i B\u00ecnh D\u01b0\u01a1ng (NACA), T\u1ed5 ch\u1ee9c L\u01b0\u01a1ng th\u1ef1c v\u00e0 N\u00f4ng nghi\u1ec7p Li\u00ean hi\u1ec7p qu\u1ed1c (FAO) v\u00e0 T\u1ed5 ch\u1ee9c Th\u00fa y Th\u1ebf gi\u1edbi (OIE) \u0111\u00e3 \u0111\u01b0a ra c\u1ea3nh b\u00e1o v\u1ec1 s\u1ef1 b\u00f9ng ph\u00e1t v\u00e0 l\u00e2y lan c\u1ee7a d\u1ecbch b\u1ec7nh do Tilapia lake virus (TiLV) g\u00e2y ra tr\u00ean c\u00e1 r\u00f4 phi. <\/p>\n\n\n\n

D\u1ea5u hi\u1ec7u nh\u1eadn bi\u1ebft<\/strong><\/p>\n\n\n\n

M\u1ed9t s\u1ed1 \u0111\u1eb7c \u0111i\u1ec3m d\u1ecbch t\u1ec5<\/strong><\/em><\/p>\n\n\n\n

Virus TiLV g\u00e2y b\u1ec7nh \u1edf c\u00e1c lo\u00e0i c\u00e1 r\u00f4 phi nu\u00f4i, g\u1ed3m: C\u00e1 r\u00f4 phi v\u1eb1n (Nile tilapia, Oreochromis niloticus), c\u00e1 r\u00f4 phi lai t\u1ea1o (O. niloticus \u00d7 O. aureus hybrids) v\u00e0 c\u00e1 r\u00f4 phi \u0111\u1ecf\/c\u00e1 \u0111i\u00eau h\u1ed3ng (Oreochromis <\/em>sp.). C\u00e1c lo\u00e0i c\u00e1 r\u00f4 phi hoang d\u00e3 nh\u01b0 c\u00e1 r\u00f4 phi xanh (Oreochromis aureus<\/em>), c\u00e1 r\u00f4 phi Mango (Sarotherodon galilaeus<\/em>), Tilapia zilli<\/em> v\u00e0 Tristamellasimonis intermedia<\/em> c\u0169ng m\u1eabn c\u1ea3m v\u1edbi vi r\u00fat n\u00e0y.<\/p>\n\n\n\n

T\u1ef7 l\u1ec7 ch\u1ebft trong c\u00e1c \u1ed5 d\u1ecbch t\u1ef1 nhi\u00ean 9 – 90%; b\u1ec7nh xu\u1ea5t hi\u1ec7n \u1edf m\u1ecdi l\u1ee9a tu\u1ed5i nh\u01b0ng ch\u1ee7 y\u1ebfu t\u1eadp trung \u1edf c\u00e1 gi\u1ed1ng (c\u00e1 con). V\u1edbi gi\u1ed1ng c\u00e1 r\u00f4 phi \u0111\u1ecf nu\u00f4i l\u1ed3ng b\u1ecb nhi\u1ec5m b\u1ec7nh, t\u1ef7 l\u1ec7 ch\u1ebft c\u00f3 th\u1ec3 l\u00ean t\u1edbi 90% trong v\u00f2ng m\u1ed9t th\u00e1ng sau th\u1ea3.<\/p>\n\n\n\n

B\u1ec7nh l\u00e2y lan theo chi\u1ec1u ngang, t\u1eeb c\u00e1 b\u1ec7nh sang c\u00e1 kh\u1ecfe trong c\u00f9ng ao nu\u00f4i, tr\u1ea1i nu\u00f4i, qua ngu\u1ed3n n\u01b0\u1edbc, d\u1ee5ng c\u1ee5\u2026<\/p>\n\n\n\n

D\u1ea5u hi\u1ec7u b\u1ec7nh l\u00fd<\/strong><\/em><\/p>\n\n\n\n

C\u00e1 m\u1eafc b\u1ec7nh c\u00f3 bi\u1ec3u hi\u1ec7n ch\u00e1n \u0103n, m\u00e0u s\u1eafc c\u01a1 th\u1ec3 bi\u1ebfn \u0111\u1ed5i (s\u1eabm m\u00e0u); thay \u0111\u1ed5i t\u1eadp t\u00ednh b\u01a1i l\u1ed9i (nh\u01b0 t\u1eadp trung \u1edf tr\u00ean b\u1ec1 m\u1eb7t, b\u01a1i l\u1edd \u0111\u1edd), ng\u1eebng k\u00e9o \u0111\u00e0n, h\u00f4n m\u00ea tr\u01b0\u1edbc khi ch\u1ebft. M\u1ed9t s\u1ed1 d\u1ea5u hi\u1ec7u tr\u00ean c\u01a1 th\u1ec3 g\u1ed3m: Hi\u1ec7n t\u01b0\u1ee3ng xung huy\u1ebft, xu\u1ea5t huy\u1ebft n\u00e3o; \u0103n m\u00f2n v\u00e0 l\u1edf lo\u00e9t t\u1eeb d\u1ea1ng \u0111i\u1ec3m \u0111\u1ebfn m\u1ea3ng tr\u00ean da; mang t\u00e1i nh\u1ee3t; m\u1eaft b\u1ecb teo l\u1ea1i ho\u1eb7c l\u1ed3i ra, c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng \u0111\u1ee5c th\u1ee7y tinh th\u1ec3; xoang b\u1ee5ng v\u00e0 h\u1eadu m\u00f4n ph\u00ecnh to; v\u1ea9y d\u1ef1ng l\u00ean, c\u00f3 th\u1ec3 bong tr\u00f3c; \u0111u\u00f4i b\u1ecb \u0103n m\u00f2n.<\/p>\n\n\n\n

B\u1ec7nh g\u00e2y t\u1ef7 l\u1ec7 ch\u1ebft cao trong \u0111\u00e0n c\u00e1 nu\u00f4i, \u0111\u1eb7c bi\u1ec7t \u1edf c\u00e1 nh\u1ecf, do \u0111\u00f3 khi th\u1ea5y c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng c\u00e1 r\u00f4 phi nu\u00f4i ch\u1ebft nhi\u1ec1u, kh\u00f4ng r\u00f5 nguy\u00ean nh\u00e2n th\u00ec c\u00f3 th\u1ec3 nghi \u0111\u00e2y l\u00e0 b\u1ec7nh do virus TiLV g\u00e2y ra.<\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p thu m\u1eabu ch\u1ea9n \u0111o\u00e1n, x\u00e9t nghi\u1ec7m<\/strong><\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p thu m\u1eabu<\/strong><\/em><\/p>\n\n\n\n

Thu nguy\u00ean \u1ea5u tr\u00f9ng, c\u00e1 b\u1ed9t, c\u00e1 gi\u1ed1ng c\u00f3 bi\u1ec3u hi\u1ec7n b\u1ec7nh l\u00fd b\u1ea5t th\u01b0\u1eddng; t\u00f9y theo k\u00edch c\u1ee1 c\u00e1, c\u00f3 th\u1ec3 thu 10 – 30 con\/ao, b\u1ec3; c\u00e1 gi\u1ed1ng nh\u1ecf, c\u00f3 th\u1ec3 g\u1ed9p 5 con th\u00e0nh m\u1ed9t m\u1eabu x\u00e9t nghi\u1ec7m; \u0111\u1ed1i v\u1edbi c\u00e1 c\u00f3 k\u00edch th\u01b0\u1edbc l\u1edbn, thu gan, th\u1eadn, l\u00e1ch, n\u00e3o v\u00e0 chuy\u1ec3n v\u1ec1 ph\u00f2ng th\u00ed nghi\u1ec7m \u0111\u1ec3 s\u1eed d\u1ee5ng trong x\u00e9t nghi\u1ec7m b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p PCR (c\u00f3 th\u1ec3 t\u00e1ch ri\u00eang t\u1eebng c\u01a1 quan ho\u1eb7c g\u1ed9p 2 – 3 c\u01a1 quan th\u00e0nh m\u1ed9t m\u1eabu \u0111\u1ec3 x\u00e9t nghi\u1ec7m). M\u1eabu d\u00f9ng trong ch\u1ea9n \u0111o\u00e1n PCR c\u00f3 th\u1ec3 l\u00e0 m\u1eabu t\u01b0\u01a1i ho\u1eb7c \u0111\u00e3 c\u1ed1 \u0111\u1ecbnh b\u1eb1ng c\u1ed3n 95% ho\u1eb7c trong dung d\u1ecbch RNAlater (QIAGEN). G\u1eedi m\u1eabu c\u00e1 s\u1ed1ng ho\u1eb7c b\u1ea3o qu\u1ea3n l\u1ea1nh \u0111\u1ebfn c\u00e1c ph\u00f2ng th\u1eed nghi\u1ec7m c\u00f3 \u0111\u1ee7 n\u0103ng l\u1ef1c x\u00e9t nghi\u1ec7m b\u1ec7nh n\u00e0y.<\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p ch\u1ea9n \u0111o\u00e1n<\/strong><\/em><\/p>\n\n\n\n

Ch\u1ea9n \u0111o\u00e1n l\u00e2m s\u00e0ng: Ch\u1ea9n \u0111o\u00e1n d\u1ef1a tr\u00ean c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m d\u1ecbch t\u1ec5 v\u00e0 d\u1ea5u hi\u1ec7u b\u1ec7nh nh\u01b0 m\u00f4 t\u1ea3 \u1edf tr\u00ean, \u0111\u1eb7c bi\u1ec7t ch\u00fa \u00fd khi c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng c\u00e1 r\u00f4 phi ch\u1ebft nhi\u1ec1u b\u1ea5t th\u01b0\u1eddng.<\/p>\n\n\n\n

Ch\u1ea9n \u0111o\u00e1n ph\u00f2ng th\u00ed nghi\u1ec7m: S\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p semi-nested PCR, c\u00f3 th\u1ec3 cho ph\u00e1t hi\u1ec7n nhanh TiLV v\u1edbi \u0111\u1ed9 nh\u1ea1y cao 7,5 copies\/ph\u1ea3n \u1ee9ng PCR v\u00e0 h\u1ea1n ch\u1ebf cho k\u1ebft qu\u1ea3 d\u01b0\u01a1ng t\u00ednh gi\u1ea3.<\/p>\n\n\n\n

Ph\u00f2ng, ch\u1ed1ng d\u1ecbch b\u1ec7nh<\/strong><\/em><\/p>\n\n\n\n

Hi\u1ec7n, ch\u01b0a c\u00f3 bi\u1ec7n ph\u00e1p ki\u1ec3m so\u00e1t hi\u1ec7u qu\u1ea3 d\u1ecbch b\u1ec7nh n\u00e0y, v\u00ec v\u1eady c\u1ea7n \u00e1p d\u1ee5ng c\u00e1c bi\u1ec7n ph\u00e1p sau khi nghi ng\u1edd ho\u1eb7c ph\u00e1t hi\u1ec7n b\u1ec7nh do TiLV g\u00e2y ra tr\u00ean c\u00e1 r\u00f4 phi, c\u1ee5 th\u1ec3:<\/p>\n\n\n\n

– N\u1ebfu c\u00f3 hi\u1ec7n t\u01b0\u1ee3ng c\u00e1 r\u00f4 phi ch\u1ebft nhi\u1ec1u b\u1ea5t th\u01b0\u1eddng, ph\u1ea3i b\u00e1o ngay cho c\u01a1 quan th\u00fa y n\u01a1i g\u1ea7n nh\u1ea5t \u0111\u1ec3 tri\u1ec3n khai k\u1ecbp th\u1eddi c\u00e1c bi\u1ec7n ph\u00e1p ph\u00f2ng ch\u1ed1ng b\u1ec7nh.<\/p>\n\n\n\n

– C\u00e1 r\u00f4 phi gi\u1ed1ng tr\u01b0\u1edbc khi th\u1ea3 nu\u00f4i c\u1ea7n \u0111\u01b0\u1ee3c l\u1ea5y m\u1eabu g\u1eedi ph\u00f2ng th\u1eed nghi\u1ec7m c\u00f3 \u0111\u1ee7 n\u0103ng l\u1ef1c \u0111\u1ec3 x\u00e9t nghi\u1ec7m s\u00e0ng l\u1ecdc \u0111\u1ed1i v\u1edbi m\u1ea7m b\u1ec7nh TiLV.<\/p>\n\n\n\n

– Tuy\u1ec7t \u0111\u1ed1i kh\u00f4ng v\u1eadn chuy\u1ec3n c\u00e1 r\u00f4 phi s\u1ed1ng t\u1eeb c\u00e1c ao nu\u00f4i \u0111\u00e3 b\u1ecb b\u1ec7nh sang c\u00e1c ao\/v\u00f9ng nu\u00f4i kh\u00f4ng b\u1ecb b\u1ec7nh \u0111\u1ec3 h\u1ea1n ch\u1ebf d\u1ecbch b\u1ec7nh l\u00e2y lan.<\/p>\n\n\n\n

– Kh\u00f4ng v\u1ee9t c\u00e1 ch\u1ebft, c\u00e1 b\u1ec7nh, x\u1ea3 th\u1ea3i n\u01b0\u1edbc ao nu\u00f4i b\u1ecb b\u1ec7nh nh\u01b0ng ch\u01b0a qua x\u1eed l\u00fd ra ngo\u00e0i m\u00f4i tr\u01b0\u1eddng l\u00e0m l\u00e2y lan d\u1ecbch b\u1ec7nh. H\u1ed1 ti\u00eau h\u1ee7y c\u00e1 ch\u1ebft ph\u1ea3i c\u00e1ch xa ngu\u1ed3n n\u01b0\u1edbc, khu d\u00e2n c\u01b0 \u00edt nh\u1ea5t 50 m. S\u1eed d\u1ee5ng v\u00f4i b\u1ed9t r\u1eafc xu\u1ed1ng h\u1ed1 v\u00e0 phun thu\u1ed1c s\u00e1t tr\u00f9ng quanh khu v\u1ef1c h\u1ed1.<\/p>\n\n\n\n

– \u00c1p d\u1ee5ng c\u00e1c bi\u1ec7n ph\u00e1p an to\u00e0n sinh h\u1ecdc, t\u0103ng c\u01b0\u1eddng qu\u1ea3n l\u00fd ao nu\u00f4i \u0111\u1ec3 h\u1ea1n ch\u1ebf m\u1ea7m b\u1ec7nh l\u00e2y lan qua d\u1ee5ng c\u1ee5, ph\u01b0\u01a1ng ti\u1ec7n v\u00e0 con ng\u01b0\u1eddi (s\u1eed d\u1ee5ng c\u00e1c bi\u1ec7n ph\u00e1p v\u1ec7 sinh, ti\u00eau \u0111\u1ed9c s\u00e1t tr\u00f9ng).<\/p>\n\n\n\n

Source:  Kim Ti\u1ebfn (T\u1ed5ng h\u1ee3p), T\u1ea1p ch\u00ed Th\u1ee7y s\u1ea3n VN<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

M\u1ea1ng l\u01b0\u1edbi c\u00e1c Trung t\u00e2m Nu\u00f4i tr\u1ed3ng th\u1ee7y s\u1ea3n khu v\u1ef1c ch\u00e2u \u00c1 – Th\u00e1i B\u00ecnh D\u01b0\u01a1ng (NACA), T\u1ed5 ch\u1ee9c L\u01b0\u01a1ng th\u1ef1c v\u00e0 N\u00f4ng nghi\u1ec7p Li\u00ean hi\u1ec7p qu\u1ed1c (FAO) v\u00e0 T\u1ed5 ch\u1ee9c Th\u00fa y Th\u1ebf gi\u1edbi (OIE) \u0111\u00e3 \u0111\u01b0a ra c\u1ea3nh b\u00e1o v\u1ec1 s\u1ef1 b\u00f9ng ph\u00e1t v\u00e0 l\u00e2y lan c\u1ee7a d\u1ecbch b\u1ec7nh do Tilapia lake virus (TiLV) g\u00e2y ra tr\u00ean c\u00e1 r\u00f4 phi. <\/p>\n","protected":false},"author":4,"featured_media":7937,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_exactmetrics_skip_tracking":false,"footnotes":""},"categories":[5],"tags":[],"class_list":["post-7936","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-thuy-san"],"yoast_head":"\nH\u01b0\u1edbng d\u1eabn ph\u00f2ng, ch\u1ed1ng b\u1ec7nh Tilapia lake virus (TiLV) tr\u00ean c\u00e1 r\u00f4 phi - 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